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Microscopic hyperspectral imaging used as a bio-optical taxonomic tool for micro- and macroalgae

Abstract

In the presented study a hyperspectral imager (400-700 nm) mounted on a stereo-microscope was used to separate differences in in vivo optical signatures identifying different pigment groups of bloom-forming phytoplankton and macroalgae by comparing spectral absorption, transmittance, and reflectance from 400-700 nm. The results show that the hyperspectral imager could be used to detect spectral characteristics on the μm level to calibrate, validate, identify, and separate objects with differences in color (optical fingerprinting). This information can be used for pigment group specific taxonomy (bio-optical taxonomy), eco-physiological information (e.g., health status), monitoring, and mapping applications.

Category

Academic article

Language

English

Author(s)

Affiliation

  • Unknown
  • SINTEF Energy Research
  • Norwegian University of Science and Technology
  • The University Centre in Svalbard

Year

2009

Published in

Applied Optics

ISSN

0003-6935

Publisher

Optical Society of America

Volume

48

Issue

21

Page(s)

4170 - 4176

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